Figure 4. Inhibition of miR-874-5p alleviated the inhibitory effect of HOTAIR knockdown on MPP+-triggered neuronal injury. SK-N-SH cells were stimulated with 1 mM MPP+ for 48 h and then transfected with si-NC, si-HOTAIR, si-HOTAIR+anti-miR-NC or si-HOTAIR+anti-miR-874-5p. (A) The expression of miR-874-5p was determined by qRT-PCR. (B) Cell viability was tested by CCK-8 assay. (C) The apoptosis rate was assessed by flow cytometry. (D) Western blot assay was conducted to detect the expression of Bcl-2 and Bax. (E-G) The levels of IL-6, IL-1β and TNF-α were measured using ELISA. (H-J) LDH activity, ROS generation and SOD activity were examined using commercial kits. *P < 0.05.
