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. 2020 Sep 30;10:16146. doi: 10.1038/s41598-020-72819-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Various histamine-releasing agents activate dog MRGPRX2 and human MRGPRX2 expressed in HEK293 cells. HEK293 cells transiently transfected with dog MRGPR family genes (D, F, G, or X2) or human MRGPRX2 were exposed to compound 48/80 or fluoroquinolones (ciprofloxacin [CPFX], gatifloxacin [GFLX], levofloxacin [LVFX], and pazufloxacin [PZFX]). (a) Increase in intracellular Ca²⁺ levels in a concentration dependent manner. Data represent the mean of quadruplicate assays. Non-transfected HEK293 cells were used as a negative control. (b) Time-course changes of intracellular Ca²⁺ levels in dog or human MRGPRX2-expressing HEK293 cells. Traces show representative intracellular Ca²⁺ fluctuation following exposure to compound 48/80 (10 μg/mL), CPFX (333 μg/mL), and PZFX (333 μg/mL). Test articles were perfused from 10 s. RLU Relative light units, s second.