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Sequences of the primer pairs for dog MRGPR family genes for RT-qPCR.
| Target gene (Accession no.) | Forward primer 5′–3′ | Reverse primer 5′–3′ |
|---|---|---|
| Dog MRGPRD (XM_540806) | GGAAGTCCTACATGGCATTG | CCAGATCACCAGGCTGTTC |
| Dog MRGPRF (NM_001313829) | GAGATGGTGGGGAACTGTTC | GAGACAAAGGAGCAGGAAGATG |
| Dog MRGPRG (NM_001313830) | CCTTCACCAACGTGCTCTTC | GAAGCCGAGGAACAGGAAG |
| Dog MRGPRX2 (XM_005633812) | GACGCTGCAGTCACAGTC | CTGGTCACTTGCATTCTTTG |
| Dog GAPDH (NM_001003142) | GGTCGGAGTGAACGGATTTG | GGAACATGTACACCATGTAGTTGAG |
