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. 2020 Sep 30;10:16112. doi: 10.1038/s41598-020-72629-9

Figure 2.

Figure 2

Effect of PM014 on the expression of fibrosis-related molecules and NF-κB activation. Immunohistostaining of TGF-β (A), IL-6 (B), and Twist (C). Quantification of the stained tissue is shown on the right. Magnification, × 10 (Scale bar 4 mm), × 40 (Scale bar 1 mm), and × 400 (Scale bar 100 μm). (D) Effect of PM014 on the mRNA levels from mouse lung tissue of TGF-β, IL-6, and Twist. (E) Activation of the NF-κB promoter by irradiation. After 24 h of transfection, the cells were subjected to 10 Gy irradiation for 6 h with or without the indicated dose of PM014 and luciferase activity was determined. (F) After 12 h of irradiation with 10 Gy, L132 cells were stained with an anti-p65 antibody to examine p65 nuclear translocation. Magnification, × 400 (Scale bar 20 μm). (G) Immunoblotting studies of NF-kB subcellular localization in irradiated L132 cells. Full length blots are presented in Supplementary Fig. S9.