TABLE 2.
An update of metagenomic xylanolytic enzymes other than GH-10 and GH-11 xylanases.
| S. No. | Source | Name | Vector/host/approaches | Library type/approach | Positive hits | OptpH | OptTemp. (°C) | Molecular weight (kDa) | Family | References |
| 1. | Yak rumen metagenome | Rubg3A and Rubg3B | pWEB Cosmid (Epicentre, United States) | Cosmid | 2/4000 | 4.5–5.5 | 35–40 | 80 | GH-3 | Bao et al., 2012 |
| 2. | Yak rumen metagenome | RuBGX1 | pWEB Cosmid (Epicentre, United States) | Cosmid | 1/5000 | 6 | 50 | 80 | GH-3 | Zhou et al., 2011 |
| 3. | Yak rumen metagenome | RuXyn1 and RuXyn2 | pWEB Cosmid (Epicentre, United States) | Cosmid | NM | 5.5–7.0 | 40–55 | 42 and 50 | GH-43 | Zhou et al., 2012 |
| 4. | Cow rumen metagenome | RUM630-BX | Lambda ZAP II Vector and Gigapack III Packaging Ext., Agilent | Lambda phage | NM | 7.0 | 25 | ND | GH-43 | Jordan et al., 2016 |
| 5. | Termite gut metagenome | Xyl1 and xyl8 | CopyControl fosmid library production kit | Fosmid | 12/8/12,000 | 5–6 | 40–50 | 53–90 | GH43 and GH-3 | Liu et al., 2018 |
| 6. | Wheat straw degrading microbial consortium | xylM1989 | Microbial consortium | NA | NA | 8 | 20 | 37.5 | GH-43 | Maruthamuthu et al., 2017 |
| 7. | Soil | MeXyl31 | CopyControl fosmid library production kit | Fosmid | 1/50,000 | 5.5 | 45 | 77 | GH-31 | Matsuzawa et al., 2016 |
| 8. | Compost | Biof1_09 | CopyControl fosmid library production kit | Fosmid | 1/– | 4.5 | 50 | 12.8 | GH-43 | Sae-Lee and Boonmee, 2014 |
| 9. | Hot spring | AR19M-311-2, AR19M-311-11 and, AR19M-311-21 | Direct sequencing | NA | NA | 5 | 50–90 | GH- 1, GH-3, GH-31, and GH-43 | Sato et al., 2017 | |
| 10. | Compost starter mixer | deAX | Lambda ZAP II vector and packaged into lambda phage | Lambda ZAP II vector | NM | 5.5–7.0 | 55 | 59.1 | GH-43 | Wagschal et al., 2009 |
| 11. | Cow rumen fluid metagenome | GH6284 | Lambda ZAP Express and Gigapack II Gold Packaging Extract | Lambda phage library | 1/50,000 | 5.8 | 45 | 110.8 | GH-5 and GH-26 | Palackal et al., 2007 |
| 12. | Goats ruminal liquid metagenome | Multiple xylanolytic enzymes | CopyControl fosmid library production kit | Fosmid | 22/150,000 endo-xylanase 125/150,000 xylosidase | 5–7 | 50 | ND | GH-1, GH-5, GH-8, GH-14, and GH-43 | Duque et al., 2018 |
| 13. | Kinema soyabean metagenome | NM | Whole metagenome sequencing | NA | NA | ND | ND | ND | GH39 and GH 43 | Kumar et al., 2019 |
| 14. | Chinese holstein dairy cow rumen | UX66 | CopyControl pCClBAC vector and E. coli | BAC | 18/15,360 | 6 | 50 | 63 | GH-43 | Zhao et al., 2010 |
| 15. | Lagoon of dairy farm | Xyn8 | Lambda ZAP II Vector/lambda phage | Fosmid | – | 6–7 | 20 | 45.9 | GH-8 | Lee et al., 2006 |
NM, not mentioned; ND, not determined; NA, not applicable.