Table 3.
Putative candidate genes for the identified QTL conferring metribuzin tolerance in wheat.
| QTL name | Refv1.0 gene ID | Gene physical position | Gene function (resources) | Gene length (bp) and direction | UniProtKB gene ID | Subcellular location | Molecular function | Biological process |
|---|---|---|---|---|---|---|---|---|
| Qrcc.uwa.2AS and Qmcc.uwa.2AS | TraesCS2A01G367800 | 611347900–611356150; 8,144 bp away from DArT1095535 | Cytochrome P450 (Refv1.0; InterPro; Pfam) | 8,258+ | A0A3B6B237_WHEAT | Other locations: integral component of membrane | Uncharacterized protein; iron ion binding; monooxygenase activity | |
| Qrcc.uwa.3AL | TraesCS3A01G329100 | 574327750–574342550; 3,715 bp away from DArT2253468 | Negative regulation of translation (GO:0017148), rRNA N-glycosylase activity (GO:0030598) | 14,770+ | A0A3B6EK35_WHEAT | Other locations | Catalytic activity; toxin activity | Defense response; negative regulation of translation |
| TraesCS3A01G300400 | 534467440–534469620; overlap with DArT1091514 | Antiporter activity; xenobiotic transmembrane transporter activity (InterPro) | 2,157+ | A0A3B6EJ09_WHEAT | Other locations: integral component of membrane | Protein detoxification (multidrug and toxic compound extrusion protein) | ||
| TraesCS3A01G289400 | 517996360–517997250; 1,075 bp away from DArT4988938 | Transcriptional repressor – ovate; (Refv1.0; InterPro) | 891+ | A0A3B6EKS8_WHEAT | Negative regulation of transcription | |||
| Qrcc.uwa.3BL | TraesCS3B01G303100 | 487203293–487224910; overlap with DArT3034325 | Ion transport protein; ion channel activity (Refv1.0; InterPro) | 21,618+ | A0A077RUK6_WHEAT | Golgi apparatus; vacuole; other locations: integral component of membrane | Uncharacterized protein; calcium ion binding; identical protein binding; ion channel activity | Ion transport; calcium-mediated signaling; regulation of jasmonic acid biosynthetic process |
| Qrcc.uwa.4AS | TraesCS4A01G452300 | 717296015–717297935; overlap with DArT1765928 | F-box-like domain superfamily (IPR036047); Transmembrane protein 183 (IPR026509) (InterPro) | 1,920+ | A0A3B6I2A1_WHEAT | Uncharacterized protein | ||
| TraesCS4A01G007400 | 4775750–4779350; 1,626 bp away from DArT995180 | Polypeptide_domain (Refv1.0; InterPro; Pfam) | 3,590– | A0A3B6HML7_WHEAT | Other locations: integral component of membrane | 3-ketoacyl-CoA synthase; transferase activity | Fatty acid biosynthesis | |
| Qrcc.uwa.5AL | TraesCS5A01G377600 | 575323152–575328643; 1677 bp away from DArT1101715 | Auxin canalization, plant pleckstrin homology-like region (Refv1.0) | 5,492+ | A0A3B6KPS3_WHEAT | Uncharacterized protein | ||
| Qvs.uwa.6AL, Qrcc.uwa.6AL, and Qmcc.uwa.6AL | TraesCS6A01G222800 | 416654584–416658623; overlap with DArT3957857 | Aromatic amino acid lyase (Refv1.0; InterPro; Pfam) | 4,040+ | A0A3B6NRY8_WHEAT | Cytoplasm | Phenylalanine ammonia-lyase, catalytic activity | Trans-cinnamate biosynthesis |
+/− represent direction (forward/reverse) on the strand; bp, base pairs.