Fig. 4. Overexpression of PpINH1 inhibits VIN activity in vivo.

Peach fruits in the green ripening stage are shown at 0 h (a) and 36 h (b) after injection with Agrobacterium to obtain transient expression of PpINH1. c Histochemical GUS staining of peach tissue from fruits injected with pBI121-PpINH1-GV3101 or pBI121-GV3101. The images were obtained 36 h after injection. The samples were imaged separately, and the images were combined to create a collage. d and e Effects of transiently overexpressed PpINH1 on the expression of PpINH1 (d) and VIN activity (e) in peach at 0, 24, 36, 48, and 72 h after injection with Agrobacterium. The values are the means ± SEs, n = 3; the lowercase letters show significant differences at the 0.05 level according to Tukey’s LSD analysis