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. 2020 Sep 30;11(9):818. doi: 10.1038/s41419-020-03017-4

Fig. 3. Nam decreases MM cell viability and induces their apoptosis.

Fig. 3

a, b MM cells were incubated with Nam for 24 h (a) and 72 h (b) followed by MTT assay. c MM cell lines were treated with DMSO (DM), Nam (4 µM) or doxorubicin (Dox, 1 µM) for 24 h followed by IB analyses. d LP1 and RPMI-8226 cells were treated with Nam for 24 h, followed by IB assay. e MM cells were treated with Dox (1 µM) or Nam for 24 h, followed by Caspase-3 (Cas-3) cleavage analysis. f LP1 and RPMI-8226 cells were treated with Nam and Z-DEVD for 24 h, followed by IB assay against Caspase-3 and PARP. g Bone marrow mononuclear cells from MM patients (n = 20) or healthy donors (n = 5) were mixed with Nam-containing MethoCultTM medium, followed by seeding in 3.5-cm plates. Colonies containing >20 cells were counted on day 14 from 20 microscopic fields. h Representative colonies from (g) were photographed. ***p < 0.001. GAPDH and β-tubulin were used as internal loading controls.