Fig. (4).

Effect of JQ1 on neuroblastoma cell lines complimented with exogenous MYCN. (A) Comparison of Relative and (B) Absolute Quantification of MYCN expression by qRT-PCR. Cell lines examined include: IMR-32 Wild Type (WT), IMR-32 Vector only control (VO), and IMR-32 cells stably selected with exogenous expression of MYCN (MYCN). MYCN expression is normalized to GAPDH in all experiments. (C) Time Course of cellular cytotoxicity in the MYCN exogenously-expressing IMR-32 cells and their appropriate controls after treatment with JQ1. Cell lines were treated with CellTox™ Green and JQ1 (8µM), and cytotoxicity levels were measured every 24 hours for 4 days. The results were normalized to samples treated only with CellTox™ Green. Data shown are the composite of sextuplicate wells, performed in triplicate from three independent measurements, with error bars representing standard deviation. (A higher resolution / colour version of this figure is available in the electronic copy of the article).