Figure 1.

circCUL3 Is Highly Expressed in GC Tissues and Correlated with Unfavorable Prognosis

(A) circRNA microarray analysis was performed to screen the circRNA expression profile as compared to the normal tissue. (B) Schematic diagram demonstrates the identification of the circular structure for circCUL3. (C) Sanger sequencing determined the genomic size and sequence of circCUL3 as reported in the circBase database (http://www.circbase.org/). (D and E) (D) qRT-PCR showed the expression level of back-spliced circCUL3 or canonical linear CUL3 mRNA treated with RNase R administration and (E) actinomycin D. (F) qRT-PCR showed the circCUL3 expression level in GC cell lines and normal cells. (G) qRT-PCR showed the circCUL3 expression level in GC tissue specimens and normal specimens. (H) Survival analysis by Kaplan-Meier survival curves showed the survival rate for GC patients with high or low circCUL3 expression. Data are shown as mean ± SD. ∗∗p < 0.01.