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. 2020 Sep 17;11:500137. doi: 10.3389/fphar.2020.500137

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Copyright © 2020 Wang, Chen, Zhao, Zhang, Bi, Zhang, Zhang, Wu and Ji

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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WCF combined with DDP affected expression and colocalization of Integrin β1and its ligand proteins(fibulin-2/laminin). (A) Immunofluorescence labeling of fibulin-2 and Integrin β1 in tumor tissue slices. (B) Immunofluorescence labeling of laminin and Integrin β1 in tumor tissue slices. (C) Mean fluorescence intensity as well as quantitative colocalization analysis of fibulin-2 and Integrin β1. (D) Mean fluorescence intensity as well as quantitative colocalization analysis of laminin and Integrin β1. Mean fluorescence intensity and Manders’coefficient were calculated by Image J. Statistical difference was analyzed by ANOVA, *P < 0.05 compared to control, ^△ P < 00.05 compared to DDP.