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. 2020 Sep 17;11:574176. doi: 10.3389/fphar.2020.574176

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Copyright © 2020 Duan, Zhu, Yang, Liu, Bai, Qu, Wang and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA expression of CYP1A2, CYP2C9, CYP2E1, CYP3A4, and UGT1A1 after treatment with siRNA-PXR and siRNA-CAR. Following transfection with the siRNA-PXR and siRNA-CAR vectors, HepG2 cells were incubated for 24 h under normoxia. The data were analyzed using a one-way analysis of variance (ANOVA), and the differences between the means of two groups were compared using LSD tests. ^* indicates the significance of experimental groups, compared with that of the control. Values were expressed as Mean ± SEM (n = 3). Levels are considered significant at ^* p < 0.05.