Table 2.
Summary report with results of a two-factor mixed-effects ANOVA and a linear mixed-effects model (see Statistical Analysis section).
| Parameter | Factor | F-value | p-value |
|---|---|---|---|
| Mixed-effects ANOVA | |||
| V cmax25 | Succ | 9.8 | 0.011 |
| J max25 | Succ | 11.6 | 0.009 |
| J max25/V cmax25 | Succ | 0.2 | 0.66 |
| A280 | Succ | 9.4 | 0.012 |
| AQY | Succ | 10.7 | 0.008 |
| R d25 | Succ | 6.9 | 0.025 |
| Ntot | Succ | 0.4 | 0.54 |
| LMA | Succ | 1.4 | 0.26 |
| Chl | Succ | 1.6 | 0.23 |
| Linear mixed-effects model | |||
| V cmax25 | Ntot | 0.67 | 0.41 |
| Succ | 8.7 | 0.015 | |
| Ntot*Succ | 5.8 | 0.018 | |
| NLH | 11.7 | 0.001 | |
| NR+B | Succ | 4.2 | 0.067 |
| NLH*Succ | 0.5 | 0.5 | |
| Ntot | 7.14 | 0.009 | |
| Rd25 | Succ | 7.14 | 0.023 |
| Ntot*Succ | 0.15 | 0.7 | |
| Ntot | LMA | 72.2 | <0.001 |
| Succ | 0.0083 | 0.93 | |
| LMA*Succ | 1.5 | 0.22 | |
| NLH | 5.7 | 0.019 | |
| AQY | Succ | 4.8 | 0.052 |
| NLH*Succ | 2 | 0.16 | |
Bold numbers represent p < 0.05. Traits analyzed were: maximum rates of Rubisco carboxylation capacity (Vcmax25, µmol m-2 s-1) and electron transport (Jmax25, µmol m-2 s-1) at 25°C; photosynthetic rates at a constant intercellular (Ci) CO2 concentration of 280 ppm (A280, µmol m-2 s-1); the ratio of Jmax25 to Vcmax25 (Jmax25:Vcmax25 ratio); leaf mass per unit leaf area (LMA, g m-2) and area-based total leaf nitrogen content (Ntot, g m-2); leaf dark respiration measured at 25˚C; apparent quantum yield of photosynthesis (AQY); fractional investments of total leaf N content into compounds maximizing photosynthetic capacity (NR+B) and compounds maximizing photosynthetic light-harvesting (NLH).