Figure 3.

PVT Inhibits Akt/Gli1 Signaling

(A–D) Western blot analysis of pAkt(Ser473), Akt, Gli1, Oct-4, SOX2, NANOG, c-Myc, cleaved caspase 3, and cleaved PARP in (A) AsPC1, (B) BxPC3, (C) MIAPaCa2, and (D) PANC1 cells treated with 5, 7.5, and 10 μM PVT for 48 h. β-Actin was used as a loading control. The figures shown are the representative blots of at least 3 independent experiments. The blots were developed using the Optimax X-Ray film processor . (E) Immunofluorescence analysis of pAkt(Ser473) (FITC, green), nucleus (DAPI, blue), phalloidin (TRITC, red) in AsPC1 cells treated with 2.5 μM PVT for 48 h, Scale bars, 200μM. (F) Gli1 activity was determined by Gli1 reporter assay in AsPC1 cells. G) Images of tumorspheres formed from PANC1 cells with or without PVT treatment. Scale bars, 1,000 μM. ∗p ≤ 0.05.