Fig. 9.
Pharmacological MPO inhibition in NASH progression affects microbiome in mice. Analysis of the gut microbiome by 16S ribosomal RNA sequencing in caecum samples of C57BL/6 mice fed with HFHC diet with (“NASH AZM198”) or without (“NASH”) MPO inhibitor, AZM198 (nNASH = 14; nNASH AZM198 = 14) (A‐C), and in samples of MPO KO and WT littermates fed with a HFCholC diet (“NASH”) for 24 weeks (nMPO WT NASH = 12; nMPO KO NASH = 16) (D‐F). (A,D) Composition of the gut microbiome represented by relative abundance of phylae. (B,E) Ratio of the phylae Firmicutes and Bacteroidetes. (C,F) Relative abundance of Verrucomicrobia species Akkermansia muciniphila. Data are presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.