FIGURE 1.

One-way approaches are utilized to analyze the three major mechanical properties including surrounding matrix, the cell’s cytoskeleton and its major the organelle the nucleus and other organelles. Hence, there are the cytoskeletal mechano-phenotype and the nuclear and other organelle mechano-phenotype. There is only one mechanical phenotype at a time that alters the matrix (blue), the cytoskeleton (yellow), and the cell nucleus and other organelles (red) in this type of approach, which is referred to as one-way approach, such as in the majority of classical migration and invasion assay.