FIG 2.

BACTH analysis of binary interactions between CesC and CesD as well as CesC and C condensation (C) domains from CesAB. Normalized β-galactosidase activity of the cells coexpressing T18-CesC and T25-CesD (A) CesC-T25 and CesA_C*-T18 (B), CesC-T18 and CesA_C-T25 (C), and CesC-T18 and CesB_C-T25 (D) expressed in Miller units (MU) is shown. The mean values from each experiment were normalized to the negative-control values (BTH101 cells coexpressing only T18 and T25 subunits of adenylate cyclase). The values of specific negative controls (only one subunit of adenylate cyclase fused with the respective target; the second subunit remained free) are also shown. Each experiment was performed at least three times independently. Error bars represent standard deviation from the mean value. ***, P < 0.0001 as determined by t test (two-sample assuming equal variances).