TABLE 1.
Bacterial strains used in this study
| Strain | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| TOP10 | Cloning host | Invitrogen |
| INV110 | Methylase-deficient cloning host | Invitrogen |
| BTH101 | Adenylate cyclase deficient (cya) reporter strain used in BACTH assay | Euromedex |
| BL21(DE3) | Expression of the proteins for the pulldown assay | Novagen |
| Tuner(DE3) | Expression of the proteins for the pulldown assay lacZY deletion mutant | Novagen |
| B. cereus | ||
| F4810/72 (AH187) | Emetic reference strain | 3 |
| F48ΔcesCD | ΔcesCD::Spcr | 31 |
| F48ΔpCER270 | pCER270 plasmid-cured strain | 31 |
| F48ΔcesCDcesCD | ΔcesCD::Spcr pAD123_PcesP_cesCD | 31 |
| F48ΔcesCDberAB | ΔcesCD::Spcr pWH1520_Pxyl berAB(Bt); Tetr | This study |
| F48ΔcesCDcesCDΔWalkerA | ΔcesCD::Spcr pAD123_PcesP_cesCDΔwalkerA; Cmr | This study |
| F48ΔcesCDcesCD_K40M | ΔcesCD::Spcr pAD123_PcesP_cesCDK40M; Cmr | This study |
| F48ΔcesCDcesCD_V29T/F30Y | ΔcesCD::Spcr pAD123_PcesP_cesCDV29T/F30Y; Cmr | This study |
| B. subtilis | ||
| 168 | Reference strain | 66 |
| Bs_cesCD/cesA_C* | amyE::[PcesP_cesC_mScarlett_cesD_T7t; Spcr], thrC::[PcesP_cesA-C*_mNeon Green; Ermr] | This study |
| Bs_cesC/cesA_C* | amyE::[PcesP_cesC_mScarlett_T7t; Spcr], thrC::[PcesP_cesA-C*_mNeon Green; Ermr] | This study |
| Bs_cesD/cesA_C* | amyE::[PcesP_cesD_T7t; Spcr], thrC::[PcesP_cesA-C*_mNeon Green; Ermr] | This study |
| B. thuringiensis 407 | Reference strain | 67 |