FIG 11.

The retromer SNX2 protein is detected in HRSV filaments. (A to C) Separate channels for HRSV F, SNX2, and HRSV M. (D) Colocalization of HRSV F and M proteins with SNX2. (E to H) Higher magnifications of panels A, B, C, and D in the area delimited in panel D. Panel H shows triple colocalization of HRSV F, SNX2, and HRSV M proteins, with arrowheads pointing to HRSV filaments and the arrow pointing to an SNX2 vesicle partially colocalizing with HRSV F protein. (I) Plot profile of the colocalizations of HRSV F, M, and SNX2 corresponding to the long arrow traced on panel H. (J) Electron microscopy of a noninfected HEp-2 cell. (K) Detail of a virus filament with SNX2 detected by immunogold labeling along the filament (arrowheads) and in the basis (arrow). (L) Higher magnification showing HRSV-like structures with the presence of SNX2 (arrowheads). The figures are representative of a single focal plan taken with a Leica SP5 microscope. Magnification, ×63. The experiment was repeated at least three times. Scale bars of immunofluorescence images = 10 μm. The electron microscopy figures were taken in a Jeol JEM-100 CXII transmission electron microscope and are representative of one experiment. The size of the scale bars of the electron microscopies are shown in the images.