FIG 3.

Effect of transfected miR-103 mimics or antagomirs on HIV-1 infection in MDMs. (A) MDMs isolated from 4 blood donors were treated with the indicated mimics or antagomirs, or controls, and infected with HIV-1 Env (ADA)- or VSV-G-pseudotyped luciferase-encoding HIV-1, and F-Luc activity was determined in the lysates 48 h following infection. RLUs were normalized to the value for F-Luc obtained in the VSV-G-pseudotyped virus-infected cells. Mean relative F-Luc activities ± SD are shown (n = 4 blood donors; Student’s t test). ns, not significant. (B) MDMs from 6 blood donors (represented by different symbols) were transfected with either controls or the indicated mimics and infected with VSV-G-pseudotyped CXCR4-tropic NL4-3. The level of HIV-1 released into the supernatant was determined after 4 days of infection by an ELISA for HIV-1 p24 (n = 6 blood donors; Student’s t test). (C) The infectivity of the released viruses in panel B was measured by determining F-Luc activity in infected TZM-bl reporter cells (n = 6 blood donors; Student’s t test). (D) Same as for panel B except that a VSV-G-pseudotyped NL4-3ΔEnv virus was used. (E) MDMs from 3 donors were transfected with either the control, the miR-103 antagomir, or the miR-103 mimic and infected with GFP-expressing HIV-1. The percentage of GFP-positive cells was determined at the indicated time points.