FIG 8.

HIV-resistant tissue-resident macrophages express high levels of miR-103/107. (A) Myeloid cells were sorted from matched sigmoid intestine biopsy specimens and blood of 3 HIV-negative study participants. The expression levels of miR-103, miR-107, and p53 mRNA in blood and colon myeloid cells were determined by real-time qPCR and normalized to the levels in blood myeloid cells (n = 3 donors, represented by different colors). (B) Fluorescence-activated cell sorting (FACS) strategy (steps 1 to 5) to obtain lung alveolar macrophages (ALVMs). DAPI, 4′,6-diamidino-2-phenylindole. SSC-A, side scatter (area); FSC-A, forward scatter (area); FSC-H, forward scatter (height). (C) ALVMs and blood monocytes were obtained from 5 HIV-positive (under ART) or 2 HIV-negative individuals, and the levels of p53 mRNA, miR-222, miR-103, and miR-107 were determined by real-time qPCR. RNA levels of ALVMs were normalized to that of the blood monocytes (n = 2 or 5 donors, represented by different colors). In all cases, bars represent the mean fold changes compared to the levels in blood myeloid cells. P values for fold changes are all <0.01 (**) compared to blood myeloid cells, from Wilcoxon matched-pairs signed-rank tests. Each dot represents a participant.