Fig. 2.
Mitochondrial respiration vs. pHcyto. (A): Representative Seahorse XF trace showing oxygen consumption rate (OCR) of mouse liver mitochondria in MRB pHcyto 6.6 (triangles) and pHcyto 7.4 (squares) sustained by succinate (state 2). ADP injection (state 3) and rotenone + antimycin A (ROT/AA) are indicated by dotted lines. Representative traces are means + Std. Dev. of one biological replicate with twelve technical replicates. (B): Oxygen consumption rates were assayed in isolated mouse liver mitochondria in succinate sustained state 2 respiration over pHcyto range 6.6–7.8. (C): Succinate + ADP sustained state 3 respiration over pHcyto range 6.6–7.8. (B and C) Data are mean OCR values relative to individual OCR values at pH 7.4 for each mitochondria preparation +SEM (N = 4) with eight-twelve technical replicates per N. One-way ANOVA with Tukey's test for multiple comparisons was performed. Student's unpaired t-test was done between all individual pHcyto values and pHcyto 7.4, values are denoted in the figure above.