Fig. 4.

Cx-I RET ROS generation vs. pHcyto. (A): ROS production with or without rotenone (+Rot) in isolated liver mitochondria incubated with succinate and ADP across a pH range 6.6–7.8. (B): Net difference between ROS generated in the absence and presence of rotenone from panel B across the pH range. (C): Calculated probability that an electron oxidized from succinate at Cx-II (Fig. 3B) will go backward and cause Cx-I RET ROS (Fig. 4B) across different values of pHcyto 6.6–7.4. Data in A and B are means + SEMs (N = 6) with six technical replicates per N. Data in C are calculated per Eqn. (1) (see text). For each applied pH (i.e. pHcyto), the equivalent pHmito is shown underneath, assuming ΔpH = 0.4 units.