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Details of primers used in this study
| Primers | Sequences (5′–3′) |
|---|---|
| E2-F | GAGCTCaATGCTCCCAGCCTGTAAACC |
| E2-R | GGGCCCAAACCGGAATTCACCTAAGGTCGTTTGTTCTGAT |
| ctxB-F | GAATTCGGTGGTGGTGGTTCTGGTGGTGGTGGTTCTbATGATTAAATTAAAATTTGGTGTTT |
| ctxB-R | GGGCCCTTAATTTGCCATACTAATTGC |
| 5′UTR-F | GGTAGCAACAGTGGTGAG |
| 5′UTR-R | GTAGCAATACAGTGGGCC |
aUnderlining indicates restriction enzyme recognize site
bItalic indicates the gene encoding flexible linker (GGGGS)2
