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. 2020 Sep 17;8:524018. doi: 10.3389/fbioe.2020.524018

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Copyright © 2020 Macedo, Martínez, Barrias and Sarmento.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment and comparison of the tight junctions’ protein ZO-1 and the mucin MUC2 in the different models at different time points. ZO-1 was labeled with Alexa-Fluor 488 (green), and MUC2 was labeled with Alexa Fluor 594 (red). The nucleus was counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue).