Figure EV1. Migration of supernumerary GnRH neurons in adulthood in the hypothalamus of mice lacking neuropilin‐1 expression in GnRH neurons.

1. Total number of GnRH neurons in the forebrain and the hypothalamus (Hypoth) and their regional distribution (DBB: diagonal band of Broca; OVLT: organum vasculosum of the lamina terminalis; 3V: periventricular area of the median preoptic) in Nrp1 ^loxP/loxP and Gnrh::cre; Nrp1 ^loxP/loxP littermates using conventional immunofluorescence. Individual males and females used for this analysis are represented by blue and gray/light‐red dots, respectively. Two‐way ANOVA, Fisher's LSD multiple‐comparison test, n = 13 (nine females and four males).
2. Representative immunohistofluorescence for GnRH (green) and Hoechst staining (blue) of sections showing the OVLT region (area framed in the schematic) from control (left) and mutant (right) adult mice. More GnRH cells are observed in OVLT sections of mutant mice (white arrow). Ac, anterior commissure; cc, corpus callosum; Scale bar: 100 μm.

Data information: Bar graphs show individual values and means ± SEM. *P < 0.05, **P < 0.01.