Figure 3. The CARD8 inflammasome is functional in human T cells.

• A
  Gene expression profile of 15 inflammasome‐related genes in human T cell and monocyte subsets. TPM values were retrieved from a public RNASeq dataset and log2 transformed. Both, rows and columns are clustered using euclidean distance and complete linkage. Tfh, T follicular helper cells; Tregs, T regulatory cells; Th, T helper cells; CM, central memory T cells; EM, effector memory T cells; TE, terminal effector T cells; CD14⁺C16⁻, classical monocytes; CD14⁺CD16⁺, intermediate monocytes; CD14⁻ CD16⁺, non‐classical monocytes.
• B
  Immunoblotting of CD4 T cells and MDMs treated with Pam3CSK4 for 6 h. * indicates an unspecific band. One representative experiment out of three is shown.
• C
  Immunoblotting of polyclonal CD4 T cells targeted with two gRNAs for each indicated target gene. One representative experiment out of three is shown.
• D
  CD4 T cells from the indicated polyclonal gene targeting approach were treated with the indicated stimuli for 18 h. Individual data points ± SEM from three independent donors are shown. Statistics indicate significance by two‐way ANOVA: ***P ≤ 0.001; ns, not significant. P‐values were corrected for multiple comparisons (Dunnett).