Skip to main content
. 2020 Aug 25;39(19):e105071. doi: 10.15252/embj.2020105071

Figure 5. TCR stimulation renders T cells resistant to pyroptosis.

Figure 5

  • A
    CD4 T cells were activated with human T activator CD3/CD28 beads (bead:cell ratio 1:5) for 4 days. Controls were kept resting in the presence of IL‐7 and IL‐15. On day 4, cells were stimulated as indicated for 18 h and cytotoxicity was assessed by LDH‐activity in the supernatant. Individual data points ± SEM from four independent donors. Statistics indicate significance by two‐way ANOVA: ***P ≤ 0.001; ns, not significant. P‐values were corrected for multiple comparisons (Sidak).
  • B
    Immunoblotting of CD4 T cell lysates treated as in (A) for 3 to 4 days. * indicates an unspecific band.
  • C
    Quantification of immunoblots shown in (B) and additional donors (n = 9 for all but GSDMD, GSDMD n = 8, for one donor quantification of the GSDMD band was considered unreliable due to uneven substrate distribution). Individual data points ± SEM are shown. Statistics indicate significance by one‐way ANOVA of log2‐transformed fold change values of band intensities (activated/non-activated T cells) normalized to β‐Actin: ***P ≤ 0.001; *P ≤ 0.05; ns, not significant. P‐values were corrected for multiple comparisons (Sidak). All band intensity ratios were tested against β‐Actin. CARD8 full length was additionally tested against CARD8 cleaved.