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. 2020 Sep 9;39(19):e105087. doi: 10.15252/embj.2020105087

Figure EV1. CtUba4 FL, AD, and RHD are dimers in solutions.

Figure EV1

  1. Tabular summary of static light‐scattering (SLS) experiments. Calculated molecular weight (MW) of the indicated proteins or protein complexes is compared to experimentally determined MWs. *For the Uba4–Urm1 complex, the MW is calculated for a 1:1 protein ratio. Selected graphs from SLS measurements are presented (right). Profiles of refractive index (RI, red), right‐angle light scattering (RALS, green), and low‐angle light scattering (LALS, black) are shown. MW: molecular weight; FL: full length; WT: wild type, AU: arbitrary units.
  2. Close‐up view of the CtUba4 RHD active loop (purple) with ball‐and-stick representation of amino acids. The second RHD molecule (grey, transparent) is shown to visualize the dimer interface. Hydrogen bonds are indicated as dashed lines.
  3. Structural comparison of the RHD active loop localization in relation to the dimeric interface of the domain in CtUba4 (cyan) and Bos taurus thiosulfate sulfurtransferase (BtTST; pink; PDB ID 1RHD).