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. 2020 Sep 9;39(19):e105087. doi: 10.15252/embj.2020105087

Figure EV2. Structural details of apo Uba4.

Figure EV2

  1. Close‐up view of the CtUba4 P‐loop-like region and the nucleotide‐binding site. Amino acids required for ATP binding are shown as ball‐and-stick model colored according to atom type. The ATP molecule (green) was modeled based on the structural similarity to the ATP‐bound EcMoeB‐MoaD (PDB ID: 1JWA).
  2. Close‐up of the CtUba4 linker (aa 286–321) showing the refined 2F o‐F c electron‐density map contoured at 1.0σ. Coordination of the zinc site is highlighted.
  3. Cartoon representation of CtUba4 and the respective structural model of ScUba4 highlighting selected mutated amino acid residues shown as black ball‐and-stick model.
  4. Interaction analysis of WT and mutated CtUba4 with GST‐CtUrm1 by GST pull‐down in the absence and presence of 1 mM ATP. Input samples (left) and samples after GST pull‐down (right) are resolved by SDS–PAGE and visualized with Coomassie stain.