Supplemental Figure S3.

Knockout (KO) mice (Lrat-Cre Pdgfra^−/− mice) have no reduction in fibrosis in bile duct ligation (BDL) or 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet models of cholestatic liver injury. A and B: No change is seen at 5 days after BDL or at 14 days after BDL as assessed by Sirius Red staining, desmin, and α-smooth muscle actin (αSMA) immunohistochemistry. Collagen was quantified by quantification of Picrosirius Red images taken with polarized light microscopy (brightfield images shown). C: KO and wild-type (WT) mice fed a DDC diet for 16 days have no differences in overall hepatic fibrosis as assessed by Sirius Red, desmin, and α-SMA immunohistochemistry. Relative collagen expression was quantified using immunofluorescence staining of collagen to avoid signal interference with intraductal porphyrin plugs. n = 3 mice at 5 days and 4 mice at 14 days per genotype (A and B); n = 5 KO mice and 7 WT mice (C). Original magnification, ×50.