Supplemental Figure S4.

Altered morphologic features and reduced midzonal distribution of hepatic stellate cells (HSCs) in knockout (KO) mice (Lrat-Cre Pdgfra^−/− mice) after bile duct ligation (BDL) and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet–induced chronic liver injury. Representative images of desmin immunohistochemistry-stained liver sections highlight reduced distribution of HSCs in midzonal areas in KO livers compared with WT controls after BDL or DDC diet–induced chronic liver injury. Original magnification: ×100 (top row); ×200 (bottom row).