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. 2020 Oct;190(10):2080–2094. doi: 10.1016/j.ajpath.2020.06.006

Figure 1.

Figure 1

Increased platelet-derived growth factor receptor (PDGFR)-α levels in animal models of fibrosis and patients with hepatic fibrosis. A: Representative Western blots showing increased expression of PDGFR-α (arrow) in whole liver lysates of mice subjected to a 4-week or 8-week course of carbon tetrachloride injections compared with corn oil–injected control mice (top left panel). A similar increase in PDGFR-β (arrow), platelet-derived growth factor (PDGF)-A, and PDGF-C is shown. Both PDGFR-α and PDGFR-β levels are significantly increased after carbon tetrachloride treatment compared with corn oil treatment as shown by densitometry on representative Western blots (top right and bottom panels). B: Western blots showing no notable differences in PDGFR-α (arrow) levels in murine livers after bile duct ligation (BDL) compared with mice undergoing sham operations. C: Confocal co-immunofluorescence imaging showing increased expression of PDGFR-α (green) co-localizing with α-smooth muscle actin (α-SMA)–positive (red) myofibroblasts in mice subjected to 8 weeks of carbon tetrachloride (middle panel) or 14 days after BDL (right panel) compared with normal liver (left panel). D: Increased PDGFR-α (arrow) levels in whole liver lysates from patients with nonalcoholic steatohepatitis (NASH) compared with normal liver tissue control. PDGFR-α levels are significantly increased in NASH cirrhotic livers compared with control livers as shown by densitometry. ∗P < 0.05, ∗∗P < 0.01 versus 8 week corn oil (non-parametric t-tests); †P < 0.05 versus normal (non-parametric t-tests). Scale bars = 100 μm. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.