Table 1.
Information on Primary Antibodies Used in the Study
| Target | Host species | Size, kDa | Western blot dilution |
IF/IHC dilution |
Diluent | Company | Catalog no. |
|---|---|---|---|---|---|---|---|
| α-SMA | Mouse | 42 | 1:100 (IF) | 0.5% BSA | Sigma-Aldrich (St. Louis, MO) | C6198 | |
| Caspase-3 | Rabbit | 17, 19, 35 | 1:1000 | 5% w/v milk | Cell Signaling Technology (Beverly, MA) | 9662 | |
| CD11b | Rat | 170 | 1:100 | PBS and 0.5% BSA | BioLegend (San Diego, CA) | 101201 | |
| Cleaved caspase-3 | Rabbit | 17, 19 | 1:1000 | 5% w/v milk | Cell Signaling Technology | 9664 | |
| CD45 | Rat | 220 | 1:100 | PBS | Santa Cruz Biotechnology, Dallas, TX | sc-53665 | |
| CD68 | Rabbit | 35 | 1:250 | PBS and 0.5% BSA | Abcam (Cambridge, UK) | Ab125212 | |
| Collagen I | Rabbit | 115,120 | 1:200 | PBS and 0.5% BSA | Abcam | Ab21286 | |
| Desmin | Rabbit | 52 | 1:200 (IHC) | 1% BSA and 1% Triton X-100 | Novus Bio (Littleton, CO) | NB120–15200 | |
| Desmin | Rabbit | 52 | 1:200 (IF) | 0.5% BSA | Thermo Fisher Scientific (Waltham, MA) | RB-9014 | |
| E-Cadherin | Rat | 35 | 1:500 (IF) | 0.5% BSA | BD Biosciences (Franklin Lakes, NJ) | BD610182 | |
| F4/80 | Rat | 160 | 1:200 (IHC) | PBS | BioRad | MCA497GA | |
| PDGF-A | Mouse | 31 | 1:200 | 5% w/v milk | Santa Cruz Biotechnology | sc-9974 | |
| PDGF-C | Goat | 30 | 1:200 | 3% BSA | R&D Systems (Minneapolis, MN) | AF1560 | |
| PDGFR-α | Rabbit | 190 | 1:1000 | 5% BSA | Cell Signaling Technology | 3174 | |
| PDGFR-α | Goat | 190 | 1:40 (IF) | 0.5% BSA | R&D Systems | AF1062 | |
| PDGFR-ß | Rabbit | 190 | 1:1000 | 5% BSA | Cell Signaling Technology | 3169 | |
| P-Y572/574-PDGFR-α/ß | Rabbit | 190 | 1:1000 | 5% BSA | Invitrogen (Carlsbad, CA) | 44-1000G | |
| P-Y742-PDGFR-α | Rabbit | 190 | 1:1000 | 5% BSA | Invitrogen | 44-1006 |
BSA, bovine serum albumin; CST, cell signaling technology; IF, immunofluorescence; IHC, immunohistochemistry; PBS, phosphate-buffered saline; PDGFR, platelet-derived growth factor receptor; α-SMA, α-smooth muscle actin.