Description of the study and laboratory testing. Analysis of the cobas MAI is not reported. AO, auramine O; DST, drug susceptibility test; INH, isoniazid; LPA, line probe assay; MAI, Mycobacterium avium and Mycobacterium intracellulare; MGIT, mycobacteria growth indicator tube; MIS, microbial inactivation solution; MTB, Mycobacterium tuberculosis; MTBDR, Mycobacterium tuberculosis drug resistant; NALC-NaOH, N-acetyl-l-cysteine sodium hydroxide; NTM, nontubercular mycobacteria; RIF, rifampicin; ZN, Ziehl-Neelsen staining.