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. 2020 Sep 22;2020:8853541. doi: 10.1155/2020/8853541

Figure 5.

Figure 5

Intracellular protein levels of MRTF-A, β-actin, α-SMA, pMLC, and MLC as a response to MRTF-A inhibition. hASCs were cultured 7 d in BM, OM, or AM media supplemented with 20 μM CCG-1423 or 12 μM CCG-100602. (a) Representative WB results of immunoblotted MRTF-A, β-actin, α-SMA, pMLC, and MLC. Semiquantitative results of MRTF-A (b), α-SMA (c), pMLC (d), and MLC (e) normalized with β-actin. MRTF-A and α-SMA: N = 2 independent experiments, 2 donors; pMLC and MLC: N = 4 independent experiments, 2 donors. BM: basic medium; OM: osteogenic medium; AM: adipogenic medium.