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. 2020 Sep 22;2020:8851525. doi: 10.1155/2020/8851525

Figure 5.

Figure 5

Cisplatin promotes translocalization of α-SMA-positive cells into SV and increases the expression of collagen IV and laminin. (a) Confocal immunofluorescence for α-SMA in lateral tissue. Nuclei were stained with DAPI (blue). Scale bars, 100 μm. (b) The distribution of collagen IV (green) in the MCs by immunofluorescence staining. After the treatment of cisplatin, the weak-to-moderate collagen IV signal was found in the SV. Nuclei were stained with DAPI (blue). Scale bars, 100 μm. (c, d) The distribution of laminin (green) in frozen sections (c) and surface preparation (d) by immunofluorescence staining. After the treatment of cisplatin, the expression of laminin increased after cisplatin treatment, blood vessel cavity became rounder, and pericytes migrated away from blood vessels. Scale bars, 100 μm. (e) Western blot of cell lysates of SV treated with cisplatin. β-Actin was used as a loading control. (g) qRT-PCR shows mRNA for laminin and collagen IV in the SV (n = 3). Con: 0.9% physiological saline; Cis: cisplatin. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.