Fig. 5. Subcellular localization and transcriptional activity analyses of CsWRKY70.

a Subcellular localization of CsWRKY70 in tobacco leaves. Agrobacterium tumefaciens carrying CsWRKY70-GFP or GFP vector (positive control) were infiltrated into tobacco leaves. The fluorescent signal was observed 60 h post infiltration. The fluorescence of CsWRKY70-GFP protein was observed only in the nucleus, while the fluorescence of the positive control was distributed throughout the whole cell. Bar = 50 μM. b Schematics of the reporter and effector constructs used in the DLR experiment. c Transcriptional activation level of CsWRKY70. The transactivation capacity of CsWRKY70 was assessed as the ratio of LUC to REN. The ratio of LUC/REN in the empty pBD vector was used as a standard and set at 1. **Significant difference between the sample and the control, P < 0.01