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. 2020 Sep 29;218(1):e20200030. doi: 10.1084/jem.20200030

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© 2020 Mishra et al.

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Figure 3. — CD8⁺ T reg cells are severely defective in Tgfbr2^−/−Eomes^−/− mice. The results from 4–5-mo-old mice are shown. (A) Representative FACS plots of pregated CD8⁺CD44^hi T cells. (B) The percentage of Ly49⁺CD122^hiCD44^hi CD8⁺ cells in total splenocytes. (C) Representative FACS plots of pregated CD8⁺Ly49⁺ T cells. (D) The percentage of Ly49⁺Helios⁺ cells in total CD8⁺ T cells. (E) The percentage of CD44^hiCD8⁺ cells in spleen. Representative results from 6 (C) or 10 (A) independent experiments are shown. Pooled results from 6 (D) or 10 (B and E) independent experiments are shown. Each symbol in B, D, and E represents the results from an individual animal. N.S., not significant; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by one-way ANOVA with Tukey multi-comparison posttest (E) or Student’s t test (B and D). WT, n = 41 (A and B), 21 (C and D), or 37 (E); Eomes^−/−, n = 13 (A and B), 12 (C and D), or 16 (E); Tgfbr2^−/−, n = 17 (A, B, and E) or 10 (C and D); and Tgfbr2^−/−Eomes^−/−, n = 22 (A and B), 10 (C and D), or 23 (E).