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. 2020 Sep 29;218(1):e20200030. doi: 10.1084/jem.20200030

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© 2020 Mishra et al.

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Figure 5. — Distinct responses of CD8⁺ vs. CD4⁺ T reg cells upon pristane challenge. (A) Gating strategy of memory-like CD8⁺ T cells (left) and the expression of Eomes and TGF-βRII on different subsets of CD8⁺ T cells isolated from WT spleen. (B) MFI of Eomes. (C) MFI of TGF-βRII on different subsets of WT CD8⁺ T cells. Representative (A) or pooled (B and C) results from four independent experiments are shown (n = 12). (D) MFI of Eomes (left) and TGF-βRII (right) on pregated CD8⁺ T reg cells. (E) The percentage of GC B cells. (F) FACS profiles of pregated CD4⁺ T cells (left) and the percentage of Foxp3⁺ within CD4⁺ T cells (right). (G) Expression of CTLA-4 (left) or GITR (right) on pregated Foxp3⁺CD4⁺ T reg cells. (H) The percentage of CD8⁺ T reg cells within CD8⁺ T cells. (I and J) The expression of Helios (I) or CXCR5 (J) on pregated CD8⁺ T reg cells. Pooled results from two independent experiments are shown in D–J. PBS group, n = 5; pristane group, n = 4. Each symbol in B–J represents the results from an individual mouse. N.S., not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 by paired (B and C) or unpaired (D–J) Student’s t test. Ctrl, control.