Figure S3.

Distinct transcription profiles of CD4⁺ vs. CD8⁺ T reg cells in WT mice. FACS-sorted CD4⁺ T reg cells (based on Foxp3-GFP; n = 2), CD8⁺ T reg cells (CD8⁺CD44^hi CD122^hiLy49⁺; n = 1), and effector (eff)/memory (mem)–like CD8⁺ T cells (CD8⁺CD44^hiCD49d⁺; n = 2) from WT mice were subjected to global RNA-seq analysis. (A) Heatmap of differentially expressed genes (DEGs). (B and C) Volcano plots of CD8⁺ T reg cells vs. effector/memory-like CD8⁺ T cells (B) or CD8⁺ T reg cells vs. CD4⁺ T reg cells (C). Numbers on the right of volcano plots indicate the total number of DEGs between the groups. DEGs between CD4⁺ and CD8⁺ T reg cells are subjected to Kyoto Encyclopedia of Genes and Genomes pathway analysis. Significantly enriched pathways are shown in D and E.