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. 2020 Sep 29;218(1):e20200030. doi: 10.1084/jem.20200030

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© 2020 Mishra et al.

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Figure S5. — Defective homeostasis of Tgfbr2^−/−Eomes^−/− CD8⁺ T reg cells. (A) Schematic of the experiments shown in B. Briefly, WT total splenocytes plus sorted CD8⁺ T reg cells carrying distinct congenic markers were cotransferred into Rag1^−/− mice and examined 14 d later. (B) The percentage of CD8⁺ T reg cells from various donor mice 14 d after transfer is shown. Each symbol in B represents the results from an individual recipient mouse (n = 3 or 4); bar graphs indicate the mean (± SEM). N.S., not significant; *, P < 0.05; ***, P < 0.001 by Student’s t test. Representative results from two independent experiments are shown.