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. 2020 Sep 30;10:16075. doi: 10.1038/s41598-020-72828-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Perturbation of Pax6 in retinal explant cultures. (A) Schematic of the methodology adopted to perform perturbation of Pax6 in retinal explant cultures. (B and C) Ex-vivo retinal explant culture indicates a significant reduction in axonal outgrowth and fasciculation in Pax6 siRNA treated explants (C, c′, c″) compared to the control (B, b′, b″). Scale bar—100 µm, B and C represent images generated by stitching multiple images using Photoshop. (D and E) Quantification of the neurite numbers (D; p < 0.05) and the length of the axons/bundles (E; t-test; p < 0.001) for the explant culture represented in B and C using ImageJ software. n = 3, 3 independent explant culture preparation were made. Error bars indicate SEM from three biological replicates.