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. 2020 Sep 30;10:16143. doi: 10.1038/s41598-020-72715-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Regular PCR of a product covering parts of 18S and ITS1 of Bonamia ostreae using gill and mantle tissue from the oysters. Lane 1–2 gill tissue from two flat oysters positive for B. ostreae. Lane 3–4 gill tissue from flat oysters from Limfjorden negative for B. ostreae, lane 5 mantle tissue from a third oyster positive for B. ostreae, lane 6 mantle tissue from a flat oyster negative for B. ostreae, lane 7 positive control sample, lane 8–9 negative control samples from B. ostreae free oysters from Jersey, lane 10–12 no template control reactions. White lines indicate where parts from the same gel (Supplementary Fig. S2) were merged.