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. 2020 Sep 30;10:16143. doi: 10.1038/s41598-020-72715-y

Table 1.

Primers, probes and references for PCR and qPCR assays used in this study. All the assays had efficiencies within 100% ± 5% and an annealing temperature of 60 °C.

Gene
Organism
Sequence read from 5′ end to 3′ end Reference

18S

B. ostreae

Forward primer:

Reverse primer:

Probe:

CCCGGCTTCTTAGAGGGACTA

ACCTGTTATTGCCCCAATCTTC

FAM-CTGTGTCTCCAGCAGAT-BHQ1

Marty et al. (2006)23

ITS1

B. ostreae

Forward primer:

Reverse primer:

Probe:

CCCTGCCCTTTGTACACACC

TCACAAAGCTTCTAAGAACGCG

FAM-GGTGAATTAGGTGGATAAGAGCGCT-BHQ1

Corbeil et al. (2006)12

ELF 1α

O. edulis

Forward primer:

Reverse primer:

Probe:

GTCACGGACAGCAAAACGTC

TCGATTGCCACACTGCTCAT

FAM-GGTGAATTAGGTGGATAAGAGCGCT-BHQ1

Present study

IAC

IAG52B

Forward primer:

Reverse primer:

Probe:

CCAGTGTATCGCCTGTCAGG

ACTGGGTGAAGGTGGGAGAT

CY5-GGCGGTGCCGGCAGGACACAGG-BHQ2

Present study

An internal amplification control assay (IAC) designed in the present study consisted of a plasmid with an artificial IAG52B construct and a CY5 labelled probe. Thus, this IAC was used in our assays using FAM labelled probes to detect inhibition in each sample.