Table 1.
Gene Organism |
Sequence read from 5′ end to 3′ end | Reference | |
---|---|---|---|
18S B. ostreae |
Forward primer: Reverse primer: Probe: |
CCCGGCTTCTTAGAGGGACTA ACCTGTTATTGCCCCAATCTTC FAM-CTGTGTCTCCAGCAGAT-BHQ1 |
Marty et al. (2006)23 |
ITS1 B. ostreae |
Forward primer: Reverse primer: Probe: |
CCCTGCCCTTTGTACACACC TCACAAAGCTTCTAAGAACGCG FAM-GGTGAATTAGGTGGATAAGAGCGCT-BHQ1 |
Corbeil et al. (2006)12 |
ELF 1α O. edulis |
Forward primer: Reverse primer: Probe: |
GTCACGGACAGCAAAACGTC TCGATTGCCACACTGCTCAT FAM-GGTGAATTAGGTGGATAAGAGCGCT-BHQ1 |
Present study |
IAC IAG52B |
Forward primer: Reverse primer: Probe: |
CCAGTGTATCGCCTGTCAGG ACTGGGTGAAGGTGGGAGAT CY5-GGCGGTGCCGGCAGGACACAGG-BHQ2 |
Present study |
An internal amplification control assay (IAC) designed in the present study consisted of a plasmid with an artificial IAG52B construct and a CY5 labelled probe. Thus, this IAC was used in our assays using FAM labelled probes to detect inhibition in each sample.