Figure 3.

Galnt2 and PC families regulate the cleavage of Angptl3 in vivo. (a) Angptl3 protein levels in serum of mice overexpressing Galnt2 or profurin. Adeno-associated virus expressing Galnt2 or profurin were infused into mice through tail vein. 14 days later, serum was collected at refeeding and was subjected to analysis as indicated in “Methods” (N = 5/group, Male, 8 weeks). NC was serum collected from Angptl3 knocking down mice and was used as negative control. Quantifications of the ANGPTL3-C versus ANGPTL3-FL or ANGPTL3-N versus ANGPTL3-FL immunoblots were shown at the right. (b) Serum levels of triglyceride and total cholesterol in mice used in A. Experiments were repeated for at least three times with similar results. *P < 0.05, **P < 0.01, ***P < 0.001.