Table 6.
Recoveries, matrix effect, and stability of the 6 alkaloids (n = 6).
| Alkaloids | Concentration (ng/mL) | Extraction recovery (%) | R.S.D. (%) | Matrix effect (%) | R.S.D. (%) | Stability (12 h at 4°C, %) | R.S.D. (%) |
|---|---|---|---|---|---|---|---|
| AC | 0.1 | 97.11 | 1.57 | 97.65 | 2.64 | 95.83 | 14.42 |
| 50 | 83.31 | 7.22 | 85.00 | 4.87 | 93.49 | 4.40 | |
| 400 | 95.75 | 6.30 | 91.42 | 6.54 | 88.65 | 7.54 | |
|
| |||||||
| MA | 0.1 | 97.76 | 5.63 | 96.91 | 4.99 | 97.17 | 8.93 |
| 50 | 86.77 | 7.48 | 89.80 | 9.13 | 88.66 | 5.70 | |
| 400 | 82.04 | 8.27 | 89.36 | 5.37 | 95.54 | 6.49 | |
|
| |||||||
| HA | 0.1 | 99.09 | 0.63 | 98.94 | 0.75 | 96.33 | 14.06 |
| 50 | 86.07 | 8.92 | 87.24 | 11.96 | 91.58 | 9.56 | |
| 400 | 82.20 | 9.43 | 86.40 | 11.18 | 96.15 | 7.48 | |
|
| |||||||
| BAC | 0.1 | 99.54 | 2.58 | 98.54 | 2.06 | 89.83 | 10.89 |
| 50 | 97.20 | 10.67 | 92.49 | 8.79 | 94.91 | 10.03 | |
| 400 | 98.79 | 8.00 | 93.48 | 6.76 | 94.14 | 4.31 | |
|
| |||||||
| BMA | 0.1 | 99.77 | 0.18 | 99.80 | 0.20 | 100.50 | 11.24 |
| 50 | 87.16 | 11.59 | 93.33 | 9.46 | 97.10 | 8.89 | |
| 400 | 96.46 | 10.05 | 95.21 | 5.20 | 98.80 | 6.86 | |
|
| |||||||
| BHA | 0.1 | 100.42 | 0.70 | 100.70 | 0.63 | 108.00 | 12.17 |
| 50 | 96.35 | 12.10 | 99.74 | 7.09 | 98.07 | 9.28 | |
| 400 | 98.74 | 3.44 | 98.25 | 1.65 | 97.91 | 1.71 | |