Table 2.
Rank of amino acids turnover (depletion/appearance) based on their discriminating power (AUC) from ROC curve analysis.
| AUC | p-value | Optimal cutoff point | Sensitivity | Specificity | Positive predictive value | Negative predictive value | Positive likelihood ratio (LR) | Negative likelihood ratio (LR) | AUC rank | |
|---|---|---|---|---|---|---|---|---|---|---|
| The proportion of 2PN (the group-IVM system) | ||||||||||
| Arg | 1.00 | < 0.000001 | − 0.60 | 100 | 100 | 100 | 100 | Inf | 0.0 | 1 |
| Gln | 0.75 | 0.09 | − 8.90 | 66.7 | 100 | 100 | 75.0 | Inf | 0.33 | 2 |
| The proportion of oocyte degeneration, by day 3 post-insemination (the group-IVM system) | ||||||||||
| Arg | 0.91 | < 0.00001 | − 0.60 | 85.7 | 100 | 100 | 83.3 | Inf | 0.14 | 1 |
| Gln | 0.74 | 0.12 | − 8.90 | 57.1 | 100 | 100 | 62.5 | Inf | 0.43 | 2 |
| The percentage of cleavage, 2–16 cell stage (the group-IVM system) | ||||||||||
| Val | 1.0 | 0.000001 | 1.16 | 100 | 100 | 100 | 100 | Inf | 0.0 | 1 |
| App. EAAs | 1.0 | 0.000001 | 7.14 | 100 | 100 | 100 | 100 | Inf | 0.0 | 2 |
| Turn. EAAs | 1.0 | 0.000001 | 7.14 | 100 | 100 | 100 | 75.0 | Inf | 0.0 | 3 |
| Arg | 0.96 | 0.000003 | − 0.17 | 88.9 | 100 | 100 | 75.0 | Inf | 0.11 | 4 |
| Ser | 0.93 | 0.000005 | − 1.65 | 88.9 | 100 | 100 | 75.0 | Inf | 0.11 | 5 |
| Trp | 0.89 | 0.0001 | 0.20 | 66.7 | 100 | 100 | 50.0 | Inf | 0.33 | 6 |
| Net. EAAs | 0.89 | 0.0001 | 7.14 | 66.7 | 100 | 100 | 50.0 | Inf | 0.33 | 7 |
| Thr | 0.89 | 0.0001 | 1.32 | 88.9 | 100 | 100 | 75.0 | Inf | 0.11 | 8 |
| Dep. Total | 0.89 | 0.0001 | − 9.39 | 88.9 | 100 | 100 | 75.0 | Inf | 0.11 | 9 |
| Net. Total | 0.85 | 0.003 | 19.43 | 100 | 66.7 | 90.0 | 100 | 3.0 | 0.0 | 10 |
| Dep. Semi | 0.85 | 0.003 | − 11.08 | 77.8 | 100 | 100 | 60.0 | Inf | 0.22 | 11 |
| Net. Semi | 0.85 | 0.003 | − 6.40 | 77.8 | 100 | 100 | 60.0 | Inf | 0.22 | 12 |
| Ile | 0.82 | 0.02 | 0.32 | 66.7 | 100 | 100 | 50 | Inf | 0.33 | 13 |
| The percentage of blastocyst formation, day 7 post-insemination (the group-IVM system) | ||||||||||
| Arg | 0.84 | 0.003 | − 0.60 | 75.0 | 100 | 100 | 66.7 | Inf | 0.25 | 1 |
| Leu | 0.84 | 0.003 | 0.97 | 87.5 | 75.0 | 87.5 | 75.0 | 3.5 | 0.17 | 2 |
| Gln | 0.84 | 0.003 | − 8.23 | 75.0 | 100 | 100 | 66.7 | Inf | 0.25 | 3 |
| Dep. Semi | 0.84 | 0.003 | − 11.18 | 75.0 | 100 | 100 | 66.7 | Inf | 0.25 | 4 |
| Ile | 0.81 | 0.01 | 0.57 | 100 | 75.0 | 88.9 | 100 | 4.0 | 0.0 | 5 |
| Thr | 0.78 | 0.04 | 1.35 | 100 | 75.0 | 88.9 | 100 | 4.0 | 0.0 | 6 |
| The proportion of the cleaved COCs, 2–16 cell stage (the individual-IVM system) | ||||||||||
| Glu | 0.76 | 0.0001 | − 0.03 | 75.0 | 83.3 | 81.8 | 76.9 | 4.5 | 0.31 | 1 |
| Ala | 0.72 | 0.003 | 2.0 | 50.0 | 100 | 100 | 66.7 | Inf | 0.52 | 2 |
| Ile | 0.71 | 0.003 | − 0.4 | 83.3 | 75.0 | 76.9 | 81.8 | 3.3 | 0.21 | 3 |
| Trp | 0.70 | 0.005 | 0.35 | 41.7 | 100 | 100 | 63.2 | Inf | 0.51 | 4 |
| Met | 0.69 | 0.009 | − 0.01 | 83.3 | 91.7 | 90.9 | 84.6 | 10.0 | 0.20 | 5 |
| Orn | 0.68 | 0.01 | 1.32 | 41.7 | 91.7 | 83.3 | 61.1 | 5.0 | 0.61 | 6 |
| Phe | 0.67 | 0.03 | − 0.08 | 75.0 | 66.7 | 69.2 | 72.7 | 2.3 | 0.37 | 7 |
| Tyr | 0.66 | 0.03 | − 0.40 | 75.0 | 66.7 | 69.2 | 72.7 | 2.3 | 0.37 | 8 |
| Arg | 0.65 | 0.05 | − 0.18 | 100 | 75.0 | 57.1 | 100 | 1.3 | 0.0 | 9 |
The ROC curve study was performed to determine the predictive power of factors (i.e., AAs) and to measure the optimal cutoff point for the positive outcome prediction. The AAs were divided into two separate groups based on the IVF outcomes of the group-IVM system (i.e., high 2PN percentage > 80%, high percentage of cleavage > 80%, high percentage of blastocyst formation > 30%; low percentage of oocyte degeneration < 15%) and the individual-IVM culture (i.e., incidence of cleavage vs. absence of cleavage).
Dep depletion, Net net balance, App appearance, Turn turnover, Total all amino acids, Non non-essential amino acids, Semi semi-essential amino acids, EAA essential amino acids, 2PN two-pronuclear zygote.