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. 2020 Sep 30;10:16117. doi: 10.1038/s41598-020-73008-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Additive effect of HMOs and KYNA on GPR35 activation. Dose response of GPR35 activation in arrestin mode to equimolar 6′SL + LNT with or without 200 µM KYNA, and to 100 µM and 200 µM KYNA, showing additive effect at concentrations between 940 µM and 2500 µM. This experiment was conducted three times, with comparable results.