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. 2020 Sep 30;10:16176. doi: 10.1038/s41598-020-73234-6

Figure 3.

Figure 3

p53 specifically binds as a tetramer to DNA containing one or two half sites. (A) AF647-p53 binds as a tetramer to wild type DNA and DNAs with a mutated or a deleted half site. The bars represent the average number of dynamic tetrameric states observed from two experiments for the wild type and mutant half site DNAs, and three experiments for the deleted half site DNA. Data with the random DNA were obtained from one experiment over four slide regions. Error bars are the range of the measurements for the wild type and mutant half site DNAs and the standard deviation of the measurements obtained with the deleted half site DNA. The p values from an unpaired two-tailed t test are as follows: wild type versus mutant 0.48, wild type versus deleted 0.07, and mutant versus deleted 0.25. (B) p53 binds to DNA lacking a p53 binding site in EMSAs. The titration of AF647-p53 was as follows (maximal tetramer concentrations): 0.075, 0.25, 0.75, 2.5, and 7.5 nM. An uncropped image of the gel is shown in Supplementary Fig. 7. (C) Tetrameric p53 does not go through a dimer/DNA intermediate en route to binding or unbinding. Shown for each DNA are the number of binding and unbinding transitions across two experiments for a total of 8 slide regions.